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Olivia Roscow

Guelph, Canada • (519) 731-3881 • spyrolivia@gmail.com

Research Scientist

I am a biochemistry, biophysics, and overall molecular biology enthusiast currently looking for work as either a Research Technician or PhD candidate in a lab that specialises in these areas. Ultimately, my mission is to aid like-minded researchers in achieving their research goals.

Contact me

Skills

DNA manipulation & analysis

  • PCR, reverse transcription, qPCR
  • Site-directed mutagenesis, standard molecular cloning techniques
  • Sanger sequencing

Protein manipulation & analysis

  • Protein extraction and purification (via both FPLC & gravity columns)
  • SDS-PAGE, gel/membrane staining, Western Blotting
  • Protein-protein interaction techniques such as ELISA, DSC, ITC, & SPR

Phage display

  • Library design and generation
  • Selection experimental design
  • Automated analysis pipeline

Cell culturing & microscopy

  • Standard bacterial, mammalian, and fish cell culturing techniques
  • Brightfield, phase contrast, and fluorescence microscopy

Computing

  • Languages — Python, Git, UNIX, R, LaTeX (interested in learning more)
  • Programs — VScode, Microsoft Office Suite, GraphPad Prism, PyMol, SnapGene, & many instrument-specific programs

Management & Leadership

  • Experimental design & project coordination
  • Data collection/management/analysis/preservation
  • Protocol troubleshooting/optimization
  • Ordering, reagent storage, grant/fund management, equipment maintenance/repair
  • Designed custom labware through modelling and 3D printing

Experience

University of Guelph

Lab Manager & Research Technician • Dec 2019 — Aug 2024

Managed protein purification, developed novel protein binders for collaborators through phage display, and assessed in vitro binding characteristics.

University of Guelph

Science Writer & Communicator • Nov 2017 — Dec 2019

Translated complex research articles to be more digestible for public audiences, specifically original research from the University of Guelph for the College of Biological Science Research Magazine.

University of Guelph

Teaching Assistant • Sep 2017 — Dec 2019

Led seminars/lab demonstrations and invigilated/marked exams and assignments for BIOL 1090 (Introduction to Molecular & Cellular Biology), MICR 2420 (Introduction to Microbiology), and MICR 3430 (Advanced Methods in Microbiology).

Pacific Biology Station

Research Technician • Sep 2016 — Jun 2017

Tested a novel anti-reoviral drug, Z-FA-FMK, on orthoreoviruses Piscine Reovirus, Chum Salmon Aquareovirus, and Infectious Hematopoietic Necrosis Virus to examine its differential effects on viruses from varied genera of the same family.

Education

University of Guelph

Master of Science, Molecular & Cellular Biology • Sep 2017 — Dec 2019

  • Queen Elizabeth II Graduate Scholarship in Science & Technology
  • Graduate Excellence Entrance Scholarship

Constructed of a full-length clone of Grapevine rupestris stem pitting-associated virus strain Syrah and developed expression/VIGS vectors for Vitis vinifera.

Vancouver Island University

Bachelor of Science, Molecular Biology • Sep 2012 — Apr 2017

  • Made the Dean's honour list every semester
  • Won 3rd place for Meritorious Best Presentation at VIU CREATE Conference

Investigated the effects of Z-FA-FMK drug treatment on aquatic reoviral and rhabdoviral replication.

Publications

  • Bodrug, T., Welsh, K., Bolhuis, D., Paulаkonis, E., Martinez-Chacin, R., Liu, B., Pinkin, N., Bonacci, T., Cui, L., Xu, P., Roscow O., Amann, S., Grishkovskaya, I., Emanuele, M., Harrison, J., Steimel, J., Hahn, K., Zhang, W., Zhong, E., Haselbach, D., Brown, N. Time-resolved cryo-EM TR-EM analysis of substrate polyubiquitination by the RING E3 anaphase-promoting complex/cyclosome APC/C. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-023-01105-5.
  • Liang, C., Roscow, O., Zhang, W. Generation and Characterization of Engineered Ubiquitin Variants to Modulate the Ubiquitin Signaling Cascade. Cold Spring Harb. Protoc. 2023. https://cshprotocols.cshlp.org/content/early/2023/03/29/pdb.over107784.abstract.
  • Xiao, H., Roscow, O., Hooker, J., Li, C., Maree, H.J., Meng, B. Concerning the Etiology of Syrah Decline: A Fresh Perspective on an Old and Complex Issue Facing the Global Grape and Wine Industry. Viruses 2023, 15, 23. https://doi.org/10.3390/v15010023.
  • Liang, C., Roscow, O., Zhang, W. Recent developments in engineering protein–protein interactions using phage display. Protein Eng. Des. Sel. 2021, 34, gzab014. https://doi.org/10.1093/protein/gzab014.
  • Roscow, O., & Zhang, W. 2021 Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases. 2021. Journal of Visualized Experiments: JoVE, 174. https://doi.org/10.3791/62950.
  • Liu, Q., Aminu, B., Roscow, O., & Zhang, W. 2021. Targeting the Ubiquitin Signaling Cascade in Tumor Microenvironment for Cancer Therapy. International Journal of Molecular Sciences, 222, 791. https://doi.org/10.3390/ijms22020791.
  • Roscow, O. 2019. Development of a Full-Length Infectious Clone of Grapevine rupestris stem pitting-associated virus strain Syrah and GFP-Tagged and VIGS Vectors for Vitis vinifera. [Thesishttps://atrium.lib.uoguelph.ca/xmlui/handle/10214/17655.
  • Roscow, O., Ganassin, R., Garver, K., & Polinski, M. 2018. Z-FA-FMK demonstrates differential inhibition of aquatic orthoreovirus PRV, aquareovirus CSRV, and rhabdovirus IHNV replication. Virus Research, 244, 194–198. https://doi.org/10.1016/j.virusres.2017.11.024.
  • Study Paves Way to Better Identify and Treat Important Pathogen. https://www.uoguelph.ca/cbs/cbs-research/research-highlights/Study_Paves_Way_to_Better_Identify_and_Treat_Important_Pathogen University of Guelph, 2019-07-25.
  • Study Shows How Agrochemicals Can Increase Risk of Parkinson’s Disease. https://www.uoguelph.ca/cbs/cbs-research/research-highlights/Study-Shows-How-Agrochemicals-Can-Increase-Risk-of-Parkinsons-Disease University of Guelph, 2019-03-29.
  • Revealing the Hidden Relationship Inside Plant Cells. https://www.uoguelph.ca/cbs/cbs-research/research-highlights/Revealing-the-Hidden-Relationships-Inside-Plant-Cells University of Guelph, 2018-06-14.
  • New Possible Drug Targets Emerge for Drug-Resistant Tuberculosis. https://www.uoguelph.ca/cbs/cbs-research/research-highlights/New-Possible-Drug-Targets-Emerge-for-Drug-Resistant-Tuberculosis University of Guelph, 2018-04-18.
  • “Junk” DNA Predicts Body Size in Seed Shrimp. https://www.uoguelph.ca/cbs/cbs-research/research-highlights/Junk-DNA-Predicts-Body-Size-in-Seed-Shrimp University of Guelph, 2018-01-10.

Projects

Primary Developer • 2021 — Present

A work-in-progress open-source Python program that facilitates quick analysis of large phage display datasets by smaller laboratories.

Social Links

  • Github: https://github.com/oroscow
  • Facebook: https://www.facebook.com/olivia.roscow/
  • LinkedIn: https://www.linkedin.com/in/oliviaroscow/
  • Instagram: https://www.instagram.com/_zhaoliya_/